Global early replication disrupts gene expression and chromatin conformation in a single cell cycle.

ABSTRACT:

Background

The early embryonic divisions of many organisms, including fish, flies, and frogs, are characterized by a very rapid S-phase caused by high rates of replication initiation. In somatic cells, S-phase is much longer due to both a reduction in the total number of initiation events and the imposition of a temporal order of origin activation. The physiological importance of changes in the rate and timing of replication initiation in S-phase remains unclear.

Results

Here we assess the importance of the temporal control of replication initiation using a conditional system in budding yeast to drive the early replication of the majority of origins in a single cell cycle. We show that global early replication disrupts the expression of over a quarter of all genes. By deleti

SUBMITTER: Santos MM 

PROVIDER: S-EPMC9575230 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature