High temporal resolution Nanopore sequencing dataset of SARS-CoV-2 and host cell RNAs.

ABSTRACT:

Background

Recent studies have disclosed the genome, transcriptome, and epigenetic compositions of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the effect of viral infection on gene expression of the host cells. It has been demonstrated that, besides the major canonical transcripts, the viral genome also codes for noncanonical RNA molecules. While the structural characterizations have revealed a detailed transcriptomic architecture of the virus, the kinetic studies provided poor and often misleading results on the dynamics of both the viral and host transcripts due to the low temporal resolution of the infection event and the low virus/cell ratio (multiplicity of infection [MOI] = 0.1) applied for the infection. It has never been tested whether the alteration in

SUBMITTER: Tombacz D 

PROVIDER: S-EPMC9575581 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature