Low Surface Potential with Glycoconjugates Determines Insect Cell Adhesion at Room Temperature.

ABSTRACT: Cell-coupled field-effect transistor (FET) biosensors have attracted considerable attention because of their high sensitivity to biomolecules. The use of insect cells (Sf21) as a core sensor element is advantageous due to their stable adhesion to sensors at room temperature. Although visualization of the insect cell-substrate interface leads to logical amplification of signals, the spatiotemporal processes at the interfaces have not yet been elucidated. We quantitatively monitored the adhesion dynamics of Sf21 using interference reflection microscopy (IRM). Specific adhesion signatures with ring-like patches along the cellular periphery were detected. A combination of zeta potential measurements and lectin staining identified specific glycoconjugates with low electrostatic potentials. The

SUBMITTER: Matsuzaki T 

PROVIDER: S-EPMC9575668 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature