Metabolic labeling of cardiomyocyte-derived small extracellular-vesicle (sEV) miRNAs identifies miR-208a in cardiac regulation of lung gene expression.

ABSTRACT: Toxoplasma gondii uracil phosphoribosyltransferase (UPRT) converts 4-thiouracil (4TUc) into 4-thiouridine (4TUd), which is incorporated into nascent RNAs and can be biotinylated, then labelled with streptavidin conjugates or isolated via streptavidin-affinity methods. Here, we generated mice that expressed T. gondii UPRT only in cardiomyocytes (^CM UPRT mice) and tested our hypothesis that CM-derived miRNAs (^CM miRs) are transferred into remote organs after myocardial infarction (MI) by small extracellular vesicles (sEV) that are released from the heart into the peripheral blood (^PB sEV). We found that 4TUd was incorporated with high specificity and sensitivity into RNAs isolated from the hearts and ^PB sEV of ^CM UPRT mice 6 h after 4TUc

SUBMITTER: Han C 

PROVIDER: S-EPMC9575700 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature