The Role of Dynamic DNA Methylation in Liver Transplant Rejection in Children.

ABSTRACT: Transcriptional regulation of liver transplant (LT) rejection may reveal novel predictive and therapeutic targets. The purpose of this article is to test the role of differential DNA methylation in children with biopsy-proven acute cellular rejection after LT.

Methods

Paired peripheral blood DNA samples were obtained before and after LT from 17 children, including 4 rejectors (Rs) and 13 nonrejectors (NRs), and assayed with MethylC capture sequencing approach covering 5 million CpGs in immune-cell-specific regulatory elements. Differentially methylated CpGs (DMCs) were identified using generalized linear regression models adjusting for sex and age and merged into differentially methylated regions (DMRs) comprising 3 or more DMCs.

Results

Contrasting Rs versus NRs, we identifi

SUBMITTER: Ningappa M 

PROVIDER: S-EPMC9575761 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature