Measurement of Residual Dipolar Couplings Using Magnetically Aligned and Flipped Nanodiscs.

ABSTRACT: Recent developments in lipid nanodisc technology have successfully overcome the major challenges in the structural and functional studies of membrane proteins and drug delivery. Among the different types of nanodiscs, the use of synthetic amphiphilic polymers created new directions including the applications of solution and solid-state NMR spectroscopy. The ability to magnetically align large-size (>20 nm diameter) polymer nanodiscs and flip them using paramagnetic lanthanide ions has enabled high-resolution studies on membrane proteins using solid-state NMR techniques. The use of polymer-based macro-nanodiscs (>20 nm diameter) as an alignment medium to measure residual dipolar couplings (RDCs) and residual quadrupole couplings by NMR experiments has also been demonstrated. In this study,

SUBMITTER: Ravula T 

PROVIDER: S-EPMC9575995 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature