Double-seedlings and embryo-free seeds generated by genetic engineering.

ABSTRACT: Apomixis can fix the heterosis of Hybrid F₁, by maintaining its heterozygous genotype, and is an ideal way for the development of hybrid rice. In this paper, we designed an engineering strategy for realizing apomictic reproduction of hybrid rice in the way of induce adventitious embryos. An embryogenesis gene, AtWUS, controlled by the ovule-specific promoter, a ribonuclease gene Barnase driven by the egg cell-specific promoter pDD45, and an inactivation gene ZmAA1 driven by the pollen-specific promoter pG47 were simultaneously integrated into one T-DNA, and co-transformed with the second T-DNA carrying a Barstar gene. Double-seedlings were observed in transgenic line. Whole-genome sequencing and ploidy levels confirmed by flow cytometry

SUBMITTER: Xia Y 

PROVIDER: S-EPMC9576183 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature