Mining and analysis of microsatellites in human coronavirus genomes using the in-house built Java pipeline.

ABSTRACT: Microsatellites or simple sequence repeats are motifs of 1 to 6 nucleotides in length present in both coding and non-coding regions of DNA. These are found widely distributed in the whole genome of prokaryotes, eukaryotes, bacteria, and viruses and are used as molecular markers in studying DNA variations, gene regulation, genetic diversity and evolutionary studies, etc. However, in vitro microsatellite identification proves to be time-consuming and expensive. Therefore, the present research has been focused on using an in-house built java pipeline to identify, analyse, design primers and find related statistics of perfect and compound microsatellites in the seven complete genome sequences of coronavirus, including the genome of coronavirus disease 2019, where the host is Homo sapiens. Base

SUBMITTER: Umang 

PROVIDER: S-EPMC9576472 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature