FTO-mediated autophagy promotes progression of clear cell renal cell carcinoma via regulating SIK2 mRNA stability.

ABSTRACT: The progression of clear cell renal cell carcinoma (ccRCC) remains a major challenge in clinical practice, and elucidation of the molecular drivers of malignancy progression is critical for the development of effective therapeutic targets. Recent studies have demonstrated that N⁶-methyladenosine (m⁶A) is the most abundant modification of eukaryotic mRNA and plays a key role in tumorigenesis and progression. However, the biological roles and underlying mechanisms of m⁶A-mediated autophagy in cancers especially in ccRCC remain poorly elucidated. m⁶A dot blot assay, m⁶A RNA methylation assay kit and immunofluorescence analysis were used to profile m⁶A levels in tissue samples and their correlation with autophagic flux. Expressi

SUBMITTER: Xu Y 

PROVIDER: S-EPMC9576516 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature