Transcellular biosynthesis of leukotriene B₄ orchestrates neutrophil swarming to fungi.

ABSTRACT: Neutrophil swarming is an emergent host defense mechanism triggered by targets larger than a single neutrophil's capacity to phagocytose. Swarming synergizes neutrophil functions, including chemotaxis, phagocytosis, and reactive oxygen species (ROS) production, and coordinates their deployment by many interacting neutrophils. The potent inflammatory lipid mediator leukotriene B₄ (LTB₄) has been established as central to orchestrating neutrophil activities during swarming. However, the details regarding how this eicosanoid choreographs the neutrophils involved in swarming are not well explained. Here we leverage microfluidics, genetically deficient mouse cells, and targeted metabolipidomic analysis to demonstrate that transcellular biosynthesis occurs among neutrophils

SUBMITTER: Hopke A 

PROVIDER: S-EPMC9576560 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature