Pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cords.

ABSTRACT: Precisely measuring the number and somatic volume of neurons in the central nervous system at single-cell resolution is technically challenging. Here, we combine multiple techniques to address this challenge in optically cleared mouse spinal cords. We describe in vivo neuron labeling approaches, tissue-clearing technology, light sheet fluorescence microscopy, and machine learning-guided imaging analysis. This combination provides a precise determination of the cell number and somatic volume of any neuron population in the spinal cords.

SUBMITTER: Yu H 

PROVIDER: S-EPMC9576748 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature