Hsa_circ_0099630 knockdown induces the proliferation and osteogenic differentiation and attenuates the apoptosis of porphyromonas gingivalis lipopolysaccharide-induced human periodontal ligament fibroblasts.

ABSTRACT:

Background

Periodontitis is an inflammatory destructive bone disease and is the most critical cause of tooth loss in adults. Recent studies have reported that circular RNAs (circRNAs) are essential in periodontitis. However, the influence and mechanism of hsa_circ_0099630 on periodontitis are not clear.

Methods

Normal periodontal tissues and inflammatory periodontal tissues were obtained from healthy patients and patients with periodontitis, respectively. Hsa_circ_0099630 was 1st identified by polymerase chain reaction (PCR) and sanger sequencing, and hsa_circ_0099630 expression was determined by real-time (RT)-quantitative PCR in periodontitis. Porphyromonas gingivalis-lipopolysaccharide (Pg-LPS) was used to construct an inflammation model in vitro. Next, cell proliferation

SUBMITTER: Wei Y 

PROVIDER: S-EPMC9577728 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature