Molecular mechanism of CD163⁺ tumor-associated macrophage (TAM)-derived exosome-induced cisplatin resistance in ovarian cancer ascites.

ABSTRACT:

Background

Studies have found that tumor-associated macrophages (TAMs) in the malignant ascites of patients with serous ovarian cancer exhibit a mixed polarization phenotype and highly variable expression of the surface marker CD163. The exosomes secreted by mesenchymal cells can be taken up by tumor cells, affecting the malignant biological behavior of them.

Methods

Using reverse transcription-polymerase chain reaction (RT-PCR) to detect the genes expression of drug resistance related factors cancer stem cells (CSCs)/multidrug resistance (MDR)/epithelial-mesenchymal transition (EMT) after CD163⁺ TAMs exosomes in ovarian cancer ascites co-cultured with A2780 and A2780/cis-diamminedichloroplatinum (DDP). Differences of the level of miR-221-3p expression between CD1

SUBMITTER: Zhang X 

PROVIDER: S-EPMC9577761 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature