Senescence-accelerated mice prone 8 (SAMP8) in male as a spontaneous osteoarthritis model.

ABSTRACT:

Background

Animal models of spontaneous osteoarthritis (OA) are sparse and not well characterized. The purpose of the present study is to examine OA-related changes and mechanisms in senescence-accelerated mouse prone 8 (SAMP8) that displays a phenotype of accelerated aging.  METHODS: Knees of male SAMP8 and SAM-resistant 1 (SAMR1) mice as control from 6 to 33 weeks of age were evaluated by histological grading systems for joint tissues (cartilage, meniscus, synovium, and subchondral bone), and µCT analysis. Gene expression patterns in articular cartilage were analyzed by real-time PCR. Immunohistochemistry was performed for OA-related factors, senescence markers, and apoptosis.

Results

Starting at 14 weeks of age, SAMP8 exhibited mild OA-like changes such as proteoglycan lo

SUBMITTER: Sanada Y 

PROVIDER: S-EPMC9578281 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature