Unambiguous identification of α-Gal epitopes in intact monoclonal antibodies by NMR spectroscopy.

ABSTRACT: The α-Gal epitope consisting of the terminal trisaccharide Galα1,3Galβ1,4GlcNAc exposed on cell or protein surfaces can cause severe immune reactions, such as hypersensitivity reactions, in humans. This epitope is also called the xenotransplantation epitope because it is one of the main reasons for the rejection of non-human organ transplants by the human innate immune response. Recombinant therapeutic proteins expressed in murine cell lines may contain α-Gal epitopes, and therefore their absence or presence needs to be tightly monitored to minimize any undesired adverse effects. The analytical identification of α-Gal epitopes in glycoproteins using the common standard techniques based on liquid chromatography and mass spectrometry is challenging, mainly due to the isobaricity of hexose st

SUBMITTER: Hinterholzer A 

PROVIDER: S-EPMC9578466 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature