Quantification of cytosol and membrane proteins in rumen epithelium of sheep with low or high CH4 emission phenotype.

ABSTRACT:

Background

Ruminant livestock are a major contributor to Australian agricultural sector carbon emissions. Variation in methane (CH4) produced from enteric microbial fermentation of feed in the reticulo-rumen of sheep differs with different digestive functions.

Method

We isolated rumen epithelium enzymatically to extract membrane and cytosol proteins from sheep with high (H) and low (L) CH4 emission. Protein abundance was quantified using SWATH-mass spectrometry.

Results

The research found differences related to the metabolism of glucose, lactate and processes of cell defence against microbes in sheep from each phenotype. Enzymes in the methylglyoxal pathway, a side path of glycolysis, resulting in D-lactate production, differed in abundance. In the H CH4 rumen epithel

SUBMITTER: Bond JJ 

PROVIDER: S-EPMC9578583 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature