Long-term morphological and functional dynamics of human stem cell-derived neuronal networks on high-density micro-electrode arrays.

ABSTRACT: Comprehensive electrophysiological characterizations of human induced pluripotent stem cell (hiPSC)-derived neuronal networks are essential to determine to what extent these in vitro models recapitulate the functional features of in vivo neuronal circuits. High-density micro-electrode arrays (HD-MEAs) offer non-invasive recording with the best spatial and temporal resolution possible to date. For 3 months, we tracked the morphology and activity features of developing networks derived from a transgenic hiPSC line in which neurogenesis is inducible by neurogenic transcription factor overexpression. Our morphological data revealed large-scale structural changes from homogeneously distributed neurons in the first month to the formation of neuronal clusters over time. This led to

SUBMITTER: Habibey R 

PROVIDER: S-EPMC9578684 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature