Identification of orphan ligand-receptor relationships using a cell-based CRISPRa enrichment screening platform.

ABSTRACT: Secreted proteins, which include cytokines, hormones, and growth factors, are extracellular ligands that control key signaling pathways mediating cell-cell communication within and between tissues and organs. Many drugs target secreted ligands and their cell surface receptors. Still, there are hundreds of secreted human proteins that either have no identified receptors ('orphans') or are likely to act through cell surface receptors that have not yet been characterized. Discovery of secreted ligand-receptor interactions by high-throughput screening has been problematic, because the most commonly used high-throughput methods for protein-protein interaction (PPI) screening are not optimized for extracellular interactions. Cell-based screening is a promising technology for the deorphanization

SUBMITTER: Siepe DH 

PROVIDER: S-EPMC9578707 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature