A quick pipeline for the isolation of 3D cell culture-derived extracellular vesicles.

ABSTRACT: Recent advances in cell biology research regarding extracellular vesicles have highlighted an increasing demand to obtain 3D cell culture-derived EVs, because they are considered to more accurately represent EVs obtained in vivo. However, there is still a grave need for efficient and tunable methodologies to isolate EVs from 3D cell cultures. Using nanofibrillar cellulose (NFC) scaffold as a 3D cell culture matrix, we developed a pipeline of two different approaches for EV isolation from cancer spheroids. A batch method was created for delivering high EV yield at the end of the culture period, and a harvesting method was created to enable time-dependent collection of EVs to combine EV profiling with spheroid development. Both these methods were easy to set up, quick to perform, and they pr

SUBMITTER: Kyykallio H 

PROVIDER: S-EPMC9579059 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature