YAP1 acts as a negative regulator of pro-tumor TAZ expression in esophageal squamous cell carcinoma.

ABSTRACT:

Purpose

Although YAP1 and TAZ are believed to be equivalent downstream effectors of the Hippo pathway, differential expression of YAP1 or TAZ suggests distinct functions during cancer progression. The exact role of YAP1 and TAZ in esophageal cancer, the 6th leading cancer-related mortality in the world, remains elusive.

Methods

Following single or double manipulation of YAP1 or TAZ expression, we subjected these manipulated cells to proliferation, migration, invasion, and xenograft tumorigenesis assays. We used RT-qPCR and Western blotting to examine their expression in the manipulated cells with or without inhibition of transcription or translation. We also examined the impact of YAP1 or TAZ deregulation on clinical outcome of esophageal cancer patients from the TCGA databa

SUBMITTER: Kuo YZ 

PROVIDER: S-EPMC9579103 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature