Genome-wide functional screening of drug-resistance genes in Plasmodium falciparum.

ABSTRACT: The global spread of drug resistance is a major obstacle to the treatment of Plasmodium falciparum malaria. The identification of drug-resistance genes is an essential step toward solving the problem of drug resistance. Here, we report functional screening as a new approach with which to identify drug-resistance genes in P. falciparum. Specifically, a high-coverage genomic library of a drug-resistant strain is directly generated in a drug-sensitive strain, and the resistance gene is then identified from this library using drug screening. In a pilot experiment using the strain Dd2, the known chloroquine-resistant gene pfcrt is identified using the developed approach, which proves our experimental concept. Furthermore, we identify multidrug-resistant transporter 7 (pfmdr7) as a novel candida

SUBMITTER: Iwanaga S 

PROVIDER: S-EPMC9579134 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature