Generation and maturation of human iPSC-derived 3D organotypic cardiac microtissues in long-term culture.

ABSTRACT: Cardiovascular diseases remain the leading cause of death worldwide; hence there is an increasing focus on developing physiologically relevant in vitro cardiovascular tissue models suitable for studying personalized medicine and pre-clinical tests. Despite recent advances, models that reproduce both tissue complexity and maturation are still limited. We have established a scaffold-free protocol to generate multicellular, beating human cardiac microtissues in vitro from hiPSCs-namely human organotypic cardiac microtissues (hOCMTs)-that show some degree of self-organization and can be cultured for long term. This is achieved by the differentiation of hiPSC in 2D monolayer culture towards cardiovascular lineage, followed by further aggregation on low-attachment culture dishes in 3D. The gener

SUBMITTER: Ergir E 

PROVIDER: S-EPMC9579206 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature