Investigation of thrombin generation assay to predict vaso-occlusive crisis in adulthood with sickle cell disease.

ABSTRACT:

Introduction

Sickle cell disease (SCD) is an inherited hemoglobinopathy disorder. The main consequence is synthesis of hemoglobin S leading to chronic hemolysis associated with morbidity. The aim of this study was to investigate Thrombin Generation Assay (TGA) to assess hypercoagulability in SCD and TGA parameters as biomarkers of vaso-occlusive crisis (VOC) risk and hospitalization within 1 year.

Materials and methods

We performed TGA in platelet poor plasma (PPP) with 1 pM of tissue factor and 4 μM of phospholipid-standardized concentration, in duplicate for patients and controls. We measured thrombomodulin (TM), soluble endothelial Protein C Receptor and Tissue Factor Pathway Inhibitor (TFPI).

Results

A total of 113 adult patients with SCD, 83 at steady state and 3

SUBMITTER: Feugray G 

PROVIDER: S-EPMC9579298 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature