Gut Epithelial Inositol Polyphosphate Multikinase Alleviates Experimental Colitis via Governing Tuft Cell Homeostasis.

ABSTRACT:

Background & aims

Inositol polyphosphate multikinase (IPMK), an essential enzyme for inositol phosphate metabolism, has been known to mediate major biological events such as growth. Recent studies have identified single-nucleotide polymorphisms in the IPMK gene associated with inflammatory bowel disease predisposition. Therefore, we aimed to investigate the functional significance of IPMK in gut epithelium.

Methods

We generated intestinal epithelial cell (IEC)-specific Ipmk knockout (IPMK^ΔIEC) mice, and assessed their vulnerability against dextran sulfate sodium-induced experimental colitis. Both bulk and single-cell RNA sequencing were performed to analyze IPMK-deficient colonic epithelial cells and colonic tuft cells.

Results

Although IPMK^ΔIEC

SUBMITTER: Park SE 

PROVIDER: S-EPMC9579329 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature