Exosomes in malignant pleural effusion from lung cancer patients impaired the cytotoxicity of double-negative T cells.

ABSTRACT: CD3⁺CD4^-CD8^- double-negative T (DNT) cells are new weapons in cancer immunotherapy. Here, we explored DNT cells in malignant pleural effusions (MPEs) from lung cancer patients. DNT cells, especially TCRαβ⁺CD56^- DNT cells, were increased in MPE from lung cancer patients. DNT cells highly expressed PD-1, TRAIL, NKG2D and DNAM-1. In contrast, FasL was barely detected in DNT cells. Compared with non-MPE cells, MPE-derived DNT cells expressed much higher levels of PD-1 and TRAIL. DNT cells from healthy peripheral blood donors potentially killed lung cancers, which was decreased by MPE supernatant. Exosomes from MPE supernatant expressed PD-1 and CEACAM1 and impaired the cytotoxicity of DNT cells. Blocking PD-1 and TIM3 rescued the cytotoxic

SUBMITTER: Wu J 

PROVIDER: S-EPMC9579705 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature