Long-term maintenance of human endometrial epithelial stem cells and their therapeutic effects on intrauterine adhesion

ABSTRACT:

Background

The human endometrium is a highly regenerative tissue that is believed to have two main types of stem cells: endometrial mesenchymal/stromal stem cells (eMSCs) and endometrial epithelial stem cells (eESCs). So far, eMSCs have been extensively studied, whereas the studies of eESCs are constrained by the inability to culture and expand them in vitro. The aim of this study is to establish an efficient method for the production of eESCs from human endometrium for potential clinical application in intrauterine adhesion (IUA).

Results

Here we developed a culture condition with a combination of some small molecules for in vitro culturing and expansion of human SSEA-1+ cells. The SSEA-1+ cells exhibited stem/progenitor cell activity in vitro, including clonogenicity an

SUBMITTER: He W 

PROVIDER: S-EPMC9580151 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature