Peripheral blood monocytes as a therapeutic target for marrow stromal cells in stroke patients

ABSTRACT:

Background

Systemic administration of marrow stromal cells (MSCs) leads to the release of a broad range of factors mediating recovery in rodent stroke models. The release of these factors could depend on the various cell types within the peripheral blood as they contact systemically administered MSCs. In this study, we assessed the immunomodulatory interactions of MSCs with peripheral blood derived monocytes (Mϕ) collected from acute stroke patients.

Methods

Peripheral blood from stroke patients was collected at 5–7 days (N = 5) after symptom onset and from age-matched healthy controls (N = 5) using mononuclear cell preparation (CPT) tubes. After processing, plasma and other cellular fractions were removed, and Mϕ were isolated from the mononuclear fraction using CD14 mic

SUBMITTER: Satani N 

PROVIDER: S-EPMC9580494 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature