Efficient Cross-Correlation Filtering of One- and Two-Color Single Molecule Localization Microscopy Data.

ABSTRACT: Single molecule localization microscopy has become a prominent technique to quantitatively study biological processes below the optical diffraction limit. By fitting the intensity profile of single sparsely activated fluorophores, which are often attached to a specific biomolecule within a cell, the locations of all imaged fluorophores are obtained with ∼20 nm resolution in the form of a coordinate table. While rendered super-resolution images reveal structural features of intracellular structures below the optical diffraction limit, the ability to further analyze the molecular coordinates presents opportunities to gain additional quantitative insights into the spatial distribution of a biomolecule of interest. For instance, pair-correlation or radial distribution functions are employed as

SUBMITTER: Mancebo A 

PROVIDER: S-EPMC9581065 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature