Modeling the gene delivery process of the needle array-based tissue nanotransfection.

ABSTRACT: Hollow needle array-based tissue nanotransfection (TNT) presents an in vivo transfection approach that directly translocate exogeneous genes to target tissues by using electric pulses. In this work, the gene delivery process of TNT was simulated and experimentally validated. We adopted the asymptotic method and cell-array-based model to investigate the electroporation behaviors of cells within the skin structure. The distribution of nonuniform electric field across the skin results in various electroporation behavior for each cell. Cells underneath the hollow microchannels of the needle exhibited the highest total pore numbers compared to others due to the stronger localized electric field. The percentage of electroporated cells within the skin structure, with pore radius over 10 nm

SUBMITTER: Li Z 

PROVIDER: S-EPMC9581438 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature