In vivo visualization of nitrate dynamics using a genetically encoded fluorescent biosensor.

ABSTRACT: Nitrate (NO₃^-) uptake and distribution are critical to plant life. Although the upstream regulation of NO₃^- uptake and downstream responses to NO₃^- in a variety of cells have been well studied, it is still not possible to directly visualize the spatial and temporal distribution of NO₃^- with high resolution at the cellular level. Here, we report a nuclear-localized, genetically encoded fluorescent biosensor, which we named NitraMeter3.0, for the quantitative visualization of NO₃^- distribution in Arabidopsis thaliana. This biosensor tracked the spatiotemporal distribution of NO₃^- along the primary root axis and disruptions by genetic mutation of transport

SUBMITTER: Chen YN 

PROVIDER: S-EPMC9581486 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature