Transcriptome Sequencing Analysis of lncRNA and mRNA Expression Profiles in Bone Nonunion.

ABSTRACT:

Background

Bone nonunion is a serious complication of fracture. This study explored the differentially expressed lncRNAs (DELs) and mRNAs (DEGs) and identified potential lncRNA-mRNA interactions in bone nonunion.

Methods

We extracted total RNA from three bone nonunion and three bone union patient tissue samples. RNA sequencing was performed to detect DELs and DEGs between bone nonunion and union tissue samples. The lncRNAs and genes with absolute log2-fold change (log2FC) > 1 and adjusted p value < 0.05 were further chosen for gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. lncRNA and targeted mRNA interaction networks were constructed.

Results

We observed 179 DELs and 415 DEGs between the bone nonunion and union tissu

SUBMITTER: Zhou J 

PROVIDER: S-EPMC9581694 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature