Deciphering imprints of impaired memory B-cell maturation in germinal centers of three patients with common variable immunodeficiency.

ABSTRACT: Common variable immunodeficiency (CVID), characterized by recurrent infections, low serum class-switched immunoglobulin isotypes, and poor antigen-specific antibody responses, comprises a heterogeneous patient population in terms of clinical presentation and underlying etiology. The diagnosis is regularly associated with a severe decrease of germinal center (GC)-derived B-cell populations in peripheral blood. However, data from B-cell differentiation within GC is limited. We present a multiplex approach combining histology, flow cytometry, and B-cell receptor repertoire analysis of sorted GC B-cell populations allowing the modeling of distinct disturbances in GCs of three CVID patients. Our results reflect pathophysiological heterogeneity underlying the reduced circulating pool of post-GC

SUBMITTER: van Schouwenburg P 

PROVIDER: S-EPMC9582261 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature