Nucleotide- and Protein-Dependent Functions of Actg1.

ABSTRACT: Cytoplasmic β- and γ-actin proteins are 99% identical but support unique organismal functions. The cytoplasmic actin nucleotide sequences Actb and Actg1, respectively, are more divergent but still 89% similar. Actb^-/- mice are embryonic lethal and Actb^-/- cells fail to proliferate, but editing the Actb gene to express γ-actin (Actb^c-g) resulted in none of the overt phenotypes of the knockout revealing protein-independent functions for Actb. To determine if Actg1 has a protein-independent function, we crossed Actb^c-g and Actg1^-/- mice to generate the bG/0 line, where the only cytoplasmic actin expressed is γ-actin from Actb^c-g. The bG/0 mice were via

SUBMITTER: Sundby LJ 

PROVIDER: S-EPMC9582642 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature