RAD18 confers radioresistance of esophagus squamous cell carcinoma through regulating p-DNA-PKcs.

ABSTRACT:

Background

Radiotherapy has recently become more common for the treatment of esophageal squamous cell carcinoma (ESCC). Radioresistance, on the other hand, continues to be a major issue because it interferes with the effectiveness of ESCC radiation. It has been demonstrated that RAD18, an E3 ubiquitin-protein ligase that regulates translesion DNA synthesis (TLS), is implicated in the regulation of genomic integrity and DNA damage response.

Methods

In the present study, immunohistochemical staining and western blotting were utilized to determine RAD18 expression in ESCC tissues and cells. ESCC cell proliferation was determined using a colony formation assay. Immunofluorescence staining, comet assay, and homologous recombination (HR)/non-homologous end-joining (NHEJ) assays we

SUBMITTER: Li X 

PROVIDER: S-EPMC9582675 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature