A microfluidic model of human vascularized breast cancer metastasis to bone for the study of neutrophil-cancer cell interactions.

ABSTRACT: The organ-specific metastatization of breast cancer to bone is driven by specific interactions between the host microenvironment and cancer cells (CCs). However, it is still unclear the role that circulating immune cells, including neutrophils, play during bone colonization (i.e. pro-tumoral vs. anti-tumoral). Here, we aimed at analyzing the migratory behavior of neutrophils when exposed to breast CCs colonizing the bone and their contribution to the growth of breast cancer micrometastases. Based on our previous bone metastasis models, we designed a microfluidic system that allows to independently introduce human vascularized breast cancer metastatic seeds within a bone-mimicking microenvironment containing osteo-differentiated mesenchymal stromal cells and endothelial cells (ECs). ECs sel

SUBMITTER: Crippa M 

PROVIDER: S-EPMC9583110 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature