Replication stress generates distinctive landscapes of DNA copy number alterations and chromosome scale losses.

ABSTRACT:

Background

A major driver of cancer chromosomal instability is replication stress, the slowing or stalling of DNA replication. How replication stress and genomic instability are connected is not known. Aphidicolin-induced replication stress induces breakages at common fragile sites, but the exact causes of fragility are debated, and acute genomic consequences of replication stress are not fully explored.

Results

We characterize DNA copy number alterations (CNAs) in single, diploid non-transformed cells, caused by one cell cycle in the presence of either aphidicolin or hydroxyurea. Multiple types of CNAs are generated, associated with different genomic regions and features, and observed copy number landscapes are distinct between aphidicolin and hydroxyurea-induced replicatio

SUBMITTER: Shaikh N 

PROVIDER: S-EPMC9583511 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature