Adenylate cyclase A amplification and functional diversification during Polyspondylium pallidum development.

ABSTRACT:

Background

In Dictyostelium discoideum (Ddis), adenylate cyclase A (ACA) critically generates the cAMP oscillations that coordinate aggregation and morphogenesis. Unlike group 4 species like Ddis, other groups do not use extracellular cAMP to aggregate. However, deletion of cAMP receptors (cARs) or extracellular phosphodiesterase (PdsA) in Polyspondylium pallidum (Ppal, group 2) blocks fruiting body formation, suggesting that cAMP oscillations ancestrally control post-aggregative morphogenesis. In group 2, the acaA gene underwent several duplications. We deleted the three Ppal aca genes to identify roles for either gene and tested whether Ppal shows transient cAMP-induced cAMP accumulation, which underpins oscillatory cAMP signalling.

Results

In contrast to Ddis, pre-aggrega

SUBMITTER: Kawabe Y 

PROVIDER: S-EPMC9583560 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature