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Cysteine Oxidation in Proteins: Structure, Biophysics, and Simulation.

ABSTRACT: Cysteine side chains can exist in distinct oxidation states depending on the pH and redox potential of the environment, and cysteine oxidation plays important yet complex regulatory roles. Compared with the effects of post-translational modifications such as phosphorylation, the effects of oxidation of cysteine to sulfenic, sulfinic, and sulfonic acid on protein structure and function remain relatively poorly characterized. We present an analysis of the role of cysteine reactivity as a regulatory factor in proteins, emphasizing the interplay between electrostatics and redox potential as key determinants of the resulting oxidation state. A review of current computational approaches suggests underdeveloped areas of research for studying cysteine reactivity through molecular simulations.

SUBMITTER: Garrido Ruiz D

PROVIDER: S-EPMC9583617 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Cysteine Oxidation in Proteins: Structure, Biophysics, and Simulation.

Garrido Ruiz Diego D Sandoval-Perez Angelica A Rangarajan Amith Vikram AV Gunderson Emma L EL Jacobson Matthew P MP

Biochemistry 20220926 20

Cysteine side chains can exist in distinct oxidation states depending on the pH and redox potential of the environment, and cysteine oxidation plays important yet complex regulatory roles. Compared with the effects of post-translational modifications such as phosphorylation, the effects of oxidation of cysteine to sulfenic, sulfinic, and sulfonic acid on protein structure and function remain relatively poorly characterized. We present an analysis of the role of cysteine reactivity as a regulator ...[more]

PMID: 36161872

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