SARS-CoV-2 Envelope Protein Forms Clustered Pentamers in Lipid Bilayers.

ABSTRACT: The SARS-CoV-2 envelope (E) protein is a viroporin associated with the acute respiratory symptoms of COVID-19. E forms cation-selective ion channels that assemble in the lipid membrane of the endoplasmic reticulum Golgi intermediate compartment. The channel activity of E is linked to the inflammatory response of the host cell to the virus. Like many viroporins, E is thought to oligomerize with a well-defined stoichiometry. However, attempts to determine the E stoichiometry have led to inconclusive results and suggested mixtures of oligomers whose exact nature might vary with the detergent used. Here, we employ ¹⁹F solid-state nuclear magnetic resonance and the centerband-only detection of exchange (CODEX) technique to determine the oligomeric number of E's transmembrane domain (

SUBMITTER: Somberg NH 

PROVIDER: S-EPMC9583936 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature