The Ultrafast and Accurate Mapping Algorithm FANSe3: Mapping a Human Whole-Genome Sequencing Dataset Within 30 Minutes.

ABSTRACT: Aligning billions of reads generated by the next-generation sequencing (NGS) to reference sequences, termed "mapping", is the time-consuming and computationally-intensive process in most NGS applications. A Fast, accurate and robust mapping algorithm is highly needed. Therefore, we developed the FANSe3 mapping algorithm, which can map a 30 × human whole-genome sequencing (WGS) dataset within 30 min, a 50 × human whole exome sequencing (WES) dataset within 30 s, and a typical mRNA-seq dataset within seconds in a single-server node without the need for any hardware acceleration feature. Like its predecessor FANSe2, the error rate of FANSe3 can be kept as low as 10^-9 in most cases, this is more robust than the Burrows-Wheeler transform-based algorithms. Error allowance hardly affec

SUBMITTER: Zhang G 

PROVIDER: S-EPMC9584123 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature