Dataset Information

Draft Genome Sequence of Streptococcus agalactiae KALRO-LC1 Strain Isolated from a Mastitis-Infected Camel in Laikipia County, Kenya.

ABSTRACT: We report the draft genome sequence of Streptococcus agalactiae KALRO-LC1 strain obtained from a mastitis-infected camel in Laikipia County, Kenya. The 2,201,604-bp draft genome is assembled into 3 contigs with a GC content of 35.87% and is predicted to contain 1,192 protein-coding sequences.

SUBMITTER: Murungi E

PROVIDER: S-EPMC9584315 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Draft Genome Sequence of Streptococcus agalactiae KALRO-LC1 Strain Isolated from a Mastitis-Infected Camel in Laikipia County, Kenya.

Murungi Edwin E Masila Ednah E Ogali Irene I Langat Nathan N Onywera Ruth R Malonza Vincent V Inguyesi Christine C Onyambu Frank F Wesonga Hezron H Maichomo Monicah M

Microbiology resource announcements 20220919 10

We report the draft genome sequence of Streptococcus agalactiae KALRO-LC1 strain obtained from a mastitis-infected camel in Laikipia County, Kenya. The 2,201,604-bp draft genome is assembled into 3 contigs with a GC content of 35.87% and is predicted to contain 1,192 protein-coding sequences. ...[more]

PMID: 36121236

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Project description:BackgroundAn estimated 59,000 people die from rabies annually, with 99% of those deaths attributable to bites from domestic dogs (Canis lupus familiaris). This preventable Neglected Tropical Disease has a large impact across continental Africa, especially for rural populations living in close contact with livestock and wildlife. Mass vaccinations of domestic dogs are effective at eliminating rabies but require large amounts of resources, planning, and political will to implement. Grassroots campaigns provide an alternative method to successful implementation of rabies control but remain understudied in their effectiveness to eliminate the disease from larger regions.Methodology/principal findingsWe report on the development, implementation, and effectiveness of a grassroots mass dog rabies vaccination campaign in Kenya, the Laikipia Rabies Vaccination Campaign. During 2015-2017, a total of 13,155 domestic dogs were vaccinated against rabies in 17 communities covering approximately 1500 km2. Based on an estimated population size of 34,275 domestic dogs, percent coverages increased across years, from 2% in 2015 to 24% in 2017, with only 3 of 38 community-years of vaccination exceeding the 70% target. The average cost of vaccinating an animal was $3.44 USD with in-kind contributions and $7.44 USD without in-kind contributions.Conclusions/significanceThe evolution of the Laikipia Rabies Vaccination Campaign from a localized volunteer-effort to a large-scale program attempting to eliminate rabies at the landscape scale provides a unique opportunity to examine successes, failures, and challenges facing grassroots campaigns. Success, in the form of vaccinating more dogs across the study area, was relatively straightforward to achieve. However, lack of effective post-vaccination monitoring and education programs, limited funding, and working in diverse community types appeared to hinder achievement of 70% coverage levels. These results indicate that grassroots campaigns will inevitably be faced with a philosophical question regarding the value of local impacts versus their contributions to a larger effort to eliminate rabies at the regional, country, or global scale.

Project description:BackgroundMastitis is a disease of economic concern that affects dairy industry worldwide. This study aimed to investigate and identify possible etiologies encountered in an episode of acute gangrenous mastitis in lactating she-camels in Al Dhafra region, Abu Dhabi Emirate, United Arab Emirates (UAE). Beside the routine clinical examination, conventional bacteriological methods were used to isolate and identify possible aerobic/anaerobic bacterial or fungal pathogens from cultured milk samples collected from the mastitic she-camels. Moreover, quantitative real-time polymerase chain reaction (qPCR) was used for the detection of Mycoplasma agalactiae and Mycoplasma bovis strains, and the 16S rRNA gene was sequenced to confirm the isolation. The isolates were also tested for their susceptibility to antimicrobials.ResultsAcute gangrenous mastitis is reported in the dromedary camel herd with about 80% morbidity rate among lactating she-camels exhibited acute, painful hard swelling of affected teat, quarter or entire udder. About 41.7% of the infected animals were stamped out for culling due to complete or partial amputation of udder quarters. Streptococcus agalactiae was the sole isolated organism (6 isolates). The antimicrobial susceptibility testing revealed that, the Streptococcus agalactiae isolates were sensitive to both penicillin and ampicillin. Comparison of the 16S rRNA gene sequencing results by BLASTN confirmed the presence of Streptococcus agalactiae with high confidence (100% identity). Phylogenetic analysis indicated clustering of one isolate (CMAUAE accession number; MN267805.1) with Streptococcus agalactiae that infects multi-hosts including humans, while strains (CMBUAE to CMFUAE with accession numbers; MN267806.1 to MN267810.1 respectively) clustered with Streptococcus agalactiae that infects humans. No Mycoplasma spp was detected by qPCR analysis.ConclusionsIn the present study, the Streptococcus agalactiae was found to be the main cause of acute gangrenous mastitis in dromedary camels in UAE. More research should be done to investigate other possible causes of clinical or subclinical mastitis in dromedary camels in UAE.

Dataset Information

Draft Genome Sequence of Streptococcus agalactiae KALRO-LC1 Strain Isolated from a Mastitis-Infected Camel in Laikipia County, Kenya.

Publications

Draft Genome Sequence of Streptococcus agalactiae KALRO-LC1 Strain Isolated from a Mastitis-Infected Camel in Laikipia County, Kenya.

Similar Datasets

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