Antisense non-coding transcription represses the PHO5 model gene at the level of promoter chromatin structure.

ABSTRACT: Pervasive transcription of eukaryotic genomes generates non-coding transcripts with regulatory potential. We examined the effects of non-coding antisense transcription on the regulation of expression of the yeast PHO5 gene, a paradigmatic case for gene regulation through promoter chromatin remodeling. A negative role for antisense transcription at the PHO5 gene locus was demonstrated by leveraging the level of overlapping antisense transcription through specific mutant backgrounds, expression from a strong promoter in cis, and use of the CRISPRi system. Furthermore, we showed that enhanced elongation of PHO5 antisense leads to a more repressive chromatin conformation at the PHO5 gene promoter, which is more slowly remodeled upon gene induction. The negative effect of antisense transcriptio

SUBMITTER: Novacic A 

PROVIDER: S-EPMC9584416 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature