Determination of oligomeric states of proteins via dual-color colocalization with single molecule localization microscopy.

ABSTRACT: The oligomeric state of plasma membrane proteins is the result of the interactions between individual subunits and an important determinant of their function. Most approaches used to address this question rely on extracting these complexes from their native environment, which may disrupt weaker interactions. Therefore, microscopy techniques have been increasingly used in recent years to determine oligomeric states in situ. Classical light microscopy suffers from insufficient resolution, but super-resolution methods such as single molecule localization microscopy (SMLM) can circumvent this problem. When using SMLM to determine oligomeric states of proteins, subunits are labeled with fluorescent proteins that only emit light following activation or conversion at different wavelengths. Typica

SUBMITTER: Tan HL 

PROVIDER: S-EPMC9584609 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature