ScRNA-sequencing reveals subtype-specific transcriptomic perturbations in DRG neurons of Pirt^EGFPf mice in neuropathic pain condition.

ABSTRACT: Functionally distinct subtypes/clusters of dorsal root ganglion (DRG) neurons may play different roles in nerve regeneration and pain. However, details about their transcriptomic changes under neuropathic pain conditions remain unclear. Chronic constriction injury (CCI) of the sciatic nerve represents a well-established model of neuropathic pain, and we conducted single-cell RNA-sequencing (scRNA-seq) to characterize subtype-specific perturbations of transcriptomes in lumbar DRG neurons on day 7 post-CCI. By using Pirt^EGFPf mice that selectively express an enhanced green fluorescent protein in DRG neurons, we established a highly efficient purification process to enrich neurons for scRNA-seq. We observed the emergence of four prominent CCI-induced clusters and a lo

SUBMITTER: Zhang C 

PROVIDER: S-EPMC9584610 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature