HIF-3α-Induced miR-630 Expression Promotes Cancer Hallmarks in Cervical Cancer Cells by Forming a Positive Feedback Loop.

ABSTRACT:

Purpose

Hypoxia has crucial functions in the development and metastasis of cervical cancer by inducing the expression of numerous genes, including microRNA genes. But we know little about how the hypoxia factors and microRNAs orchestrate to regulate hallmarks of cervical cancer cells.

Methods

We conducted RNA sequencing (RNA-seq) and chromatin immunoprecipitation sequencing (ChIP-seq) experiments to investigate the targets of HIF-3α or miR-630. ChIP-qPCR and RT-qPCR were carried out to validate the results of ChIP-seq and RNA-seq. Cellular, molecular, and radiation experiments were conducted to explore the functions of miR-630.

Results

In this study, we showed that hypoxia-induced overexpression of HIF-3α increased the expression of dozens of miRNAs, inc

SUBMITTER: Gao Q 

PROVIDER: S-EPMC9584697 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature