A high-throughput skim-sequencing approach for genotyping, dosage estimation and identifying translocations.

ABSTRACT: The development of next-generation sequencing (NGS) enabled a shift from array-based genotyping to directly sequencing genomic libraries for high-throughput genotyping. Even though whole-genome sequencing was initially too costly for routine analysis in large populations such as breeding or genetic studies, continued advancements in genome sequencing and bioinformatics have provided the opportunity to capitalize on whole-genome information. As new sequencing platforms can routinely provide high-quality sequencing data for sufficient genome coverage to genotype various breeding populations, a limitation comes in the time and cost of library construction when multiplexing a large number of samples. Here we describe a high-throughput whole-genome skim-sequencing (skim-seq) approach that can b

SUBMITTER: Adhikari L 

PROVIDER: S-EPMC9584886 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature