De novo transcriptome assembly of Conium maculatum L. to identify candidate genes for coniine biosynthesis.

ABSTRACT: Poison hemlock (Conium maculatum L.) is a notorious weed containing the potent alkaloid coniine. Only some of the enzymes in the coniine biosynthesis have so far been characterized. Here, we utilize the next-generation RNA sequencing approach to report the first-ever transcriptome sequencing of five organs of poison hemlock: developing fruit, flower, root, leaf, and stem. Using a de novo assembly approach, we derived a transcriptome assembly containing 123,240 transcripts. The assembly is deemed high quality, representing over 88% of the near-universal ortholog genes of the Eudicots clade. Nearly 80% of the transcripts were functionally annotated using a combination of three approaches. The current study focuses on describing the coniine pathway by identifying in silico transcript candidat

SUBMITTER: Peddinti G 

PROVIDER: S-EPMC9584964 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature