Genomic insights into the physiology of Quinella, an iconic uncultured rumen bacterium.

ABSTRACT: Quinella is a genus of iconic rumen bacteria first reported in 1913. There are no cultures of these bacteria, and information on their physiology is scarce and contradictory. Increased abundance of Quinella was previously found in the rumens of some sheep that emit low amounts of methane (CH₄) relative to their feed intake, but whether Quinella contributes to low CH₄ emissions is not known. Here, we concentrate Quinella cells from sheep rumen contents, extract and sequence DNA, and reconstruct Quinella genomes that are >90% complete with as little as 0.20% contamination. Bioinformatic analyses of the encoded proteins indicate that lactate and propionate formation are major fermentation pathways. The presence of a gene encoding a potential uptake hydrogenase suggests t

SUBMITTER: Kumar S 

PROVIDER: S-EPMC9585023 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature