Cell chirality exhibition of brain microvascular endothelial cells is dependent on micropattern width.

ABSTRACT: Left-right asymmetry is a conserved property in nature and observed in the human body, a property known as cell chirality. Cell chirality is often studied using micropatterned in vitro models. However, micropattern geometry and size often varies across different studies, making it challenging to compare results. Here, we utilized micropatterned RGD-peptide lines on hyaluronic acid hydrogels to investigate the effect of the micropattern width on the exhibited cell chirality bias of brain microvascular endothelial cells. Overall, this cell type exhibited a negative chirality bias on micropatterned lines ranging from 10 μm to 400 μm in width, where the negative bias was most pronounced on the 100 μm wide lines. We also observed that this exhibited chirality bias varied across the line

SUBMITTER: Porras Hernandez AM 

PROVIDER: S-EPMC9585451 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature