Development of novel DNA marker for species discrimination of Fasciola flukes based on the fatty acid binding protein type I gene.

ABSTRACT:

Background

Multiplex polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP) for nuclear phosphoenolpyruvate carboxykinase (pepck) and polymerase delta (pold), respectively, have been used to differentiate Fasciola hepatica, F. gigantica, and hybrid Fasciola flukes. However, discrimination errors have been reported in both methods. This study aimed to develop a multiplex PCR based on a novel nuclear marker, the fatty acid binding protein type I (FABP) type I gene.

Methods

Nucleotide sequence variations of FABP type I were analyzed using DNA samples of F. hepatica, F. gigantica, and hybrid Fasciola flukes obtained from 11 countries in Europe, Latin America, Africa, and Asia. A common forward primer for F. hepatica and F. gigantica and two speci

SUBMITTER: Okamoto E 

PROVIDER: S-EPMC9585863 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature